ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Prevention Of Carbon Tetrachloride-induced Oxidative Stress In Rats By Lycopene
Dr. Hülyam KURT,a Dr. Ayşe BAŞARAN,a Dr. Erinç ARALb
aTıbbi Biyoloji AD, bHistoloji Embriyoloji AD, Osmangazi Üniversitesi Tıp Fakültesi, ESKİŞEHİR
Objective: In this study, the protective effect of the antioxidant lycopene with respect to carbon tetrachloride (CCl4)-induced oxidative stress in rats was examined experimentally.
Material and Methods: In this study, thirty-two three month-old Spraque Dawley male rats were assigned to four groups in equal numbers. Group four received lycopene (5 mg/kg/day) intragastrically (i.g.) for a 10-day period as pretreatment. In the second 10-day period, group one received liquid oil (0.2 ml/kg/day) intraperitoneally (i.p.), group two CCl4 (0.2 ml/kg/day) (i.p.), and group three lycopene (5 mg/kg/day, i.g.). During this period, the previously-treated group four received the same dose of lycopene plus CCl4 daily in two divided doses. Within 24 hours of the last dose, liver tissue samples were collected for homogenate from the rats using appropriate techniques under ether anesthesia. Malondialdehide (MDA) levels were measured and catalase (CAT) as well as glutathione peroxidase (GPx) activities were determined. Tissue samples were fixed in 10% neutral formalin and slices were stained with H&E and examined under a light microscope in subsequent histological studies.
Results: Results showed, as expected, that the values obtained in the lycopene only treatment groups were close to values in the control group. In the CCl4 treated group, free radical formation secondary to CCl4 metabolism resulted in increased MDA levels and led to oxidative damage. This damage was corrected to levels comparable to those of the controls by the free radical scavengers CAT and GPx, the activities of which were noticeably increased secondary to lycopene treatments. Histological studies supported these biochemical results.
Conclusion: These results provide promising information regarding the ability of lycopene to act as a dose- and duration of use-dependant chemo-preventive or therapeutic agent.Keywords: Carbon tetrachloride, lycopene, free radicals, ratsTurkiye Klinikleri J Med Sci 2005, 25:167-173
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