ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Activity Of Glucose-6-phosphate Dehydrogenase And Glutathione Levels In Liver Lobes Of Mus Musculus
Ergül BELGE KURUTAŞ, MD,a Metin KILINÇ, MD,a Yıldız ÇAĞLAR, MDb
aDepartment of Biochemistry, Kahramanmaraş Sütçü İmam University Medical Faculty, KAHRAMANMARAŞ
bDepartment of Histology, Çukurova University Medical Faculty, ADANA
Objective: Glucose-6-phosphate dehydrogenase (G6PDH) and glutathione (GSH) are two antioxidant systems that play important roles in the cellular response to oxidative stress. G6PDH activity and GSH levels in different liver lobes of mice lend themselves particularly to comparative investigation due to the similar homology of human and mouse G6PDH and GSH enzymes.
Material and Methods: Twenty males of Mus musculus albino mice were included in the experiments. The activity of G6PDH and GSH levels in liver were measured with the aid of the Beutler method.
Results: Mean and standard deviation per g liver for lobular G6PDH activity and GSH levels were found to be as follows: 3.30 ± 1.44 U/g and 1.84 ± 0.030 µmol/g for the median lobe; 1.56 ± 0.93 U/g and 0.57 ± 0.25 µmol/g for the right lobe; 2.43 ± 1.42 U/g, 1.06 ± 0.21 µmol/g for the left lobe; and 0.41 ± 0.17 U/g, 0.05 ± 0.02 µmol/g for the caudate lobe. The highest G6PDH activity and GSHs level were found in the median and left lobes. Moreover, significant differences in G6PDH activity and GSH levels were recorded among lobes (p< 0.05).
Conclusion: G6PDH activity and GSH levels were seen to have a heterogeneous distribution in the liver lobes of male mice.Keywords: Mice, liver, glucose phosphate dehydrogenase, glutathioneTurkiye Klinikleri J Med Sci 2005, 25:149-153
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