ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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Quantitative Real Time-polymerase Chain Reaction And Gene Expression Analysis In Haematology: A Review
Dr. Hakan SAVLI,a Dr. Özden HATIRNAZb
aTıbbi Biyoloji AD, Kocaeli Üniversitesi Tıp Fakültesi, KOCAELİ
bGenetik AD, İstanbul Üniversitesi Tıp Fakültesi DETAE (Deneysel Tıp Araştırma Enstitüsü), İSTANBUL
Recent advances in molecular techniques have provided the opportunity to assess genomic alterations comprehensively and rapidly in acquired samples. New markers derived from qualitative and quantitative DNA and RNA have served to extend morphologic interpretations and have thus been increasingly integrated into the final medical diagnosis. DNA and RNA quantifications are widely used in biological and biomedical research. In the era of the Human Genome Project, quantitation of gene expression by cells is of paramount importance in the investigation of gene patterns. Quantitative real time-polymerase chain reaction (RT-PCR) technology has recently reached a level of sensitivity, accuracy and practical ease that supports its use as a routine bioinstrumentation for gene level measurement. It has evolved into the state-of-the-art technique for the quantification of nucleic acids for mutation detection, genotyping and chimerism analysis. The extremely versatile technique of quantitative RT-PCR has had an enormous impact on the life sciences and molecular medicine. In this article, details of this method are reviewed and its haematological usage is examined.Keywords: Polymerase chain reaction, hematology, gene expressionTurkiye Klinikleri J Med Sci 2004, 24:653-660
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