ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Structural Changes Induced By Cadmium Chloride And Effects Of Metallothionein On These Changes In Rat Kidney Tissue: A Light Microscopic Study
Dr. Haki KARA,a Dr. Neriman ÇOLAKOĞLU,b Dr. Aysel KÜKNER,c Dr. Enver OZANb
aFırat Üniversitesi Sağlık Yüksek Okulu,
bHistoloji Embriyoloji AD, Fırat Üniversitesi Tıp Fakültesi, ELAZIĞ
cHistoloji Embriyoloji AD, Abant İzzet Baysal Üniversitesi İzzet Baysal Tıp Fakültesi, BOLU
Objective: The aim of present study was to determine by light microscopy the cadmium chloride-induced structural changes and protective effects of co-administration of metallothionein in kidney tissue.
Material and Methods: Thirty-six adult Wistar male rats were used in this study. Animals were divided into three groups. Cadmium chloride (3.5 mg/kg/day) was subcutaneously (s.c) injected to the first group (n= 16), whereas the second group (n= 16) received both cadmium chloride (3.5 mg/kg/day s.c) and metallothionein (30 µmol/kg/day intraperitoneally). The rats in the third group (n= 4) were used as controls. On the 1st, 3rd, 5th and 7th days of the study, kidney tissue samples were taken under ether anesthesia and fixed with 10% formaldehyde solution and prepared in paraffin blocks. Tissue sections (5 µm) were stained with Hematoxylin & Eosin, Periodic Acid Schiff and Masson’s trichrom.
Results: Kidney tissue damage including basal membrane thickening of the renal corpuscule and tubules, degeneration in the proximal tubules and vascular congestion in the renal cortex were observed in both experimental groups dependent time. Moreover, in the second group, an increase in intramesangial cells, PAS (+) granules in the tubules and, in the corticomedullar tubules, an accumulation of PAS (+) hyaline substance was observed.
Conclusion: It was determined that cadmium chloride has a toxic effect in kidneys and causes serious structural damage. Metallothionein, known as a metal-binding protein, proved insufficient in preventing cadmium chloride-induced damage.Keywords: Cadmium, metallothionein, kidney, microscopeTurkiye Klinikleri J Med Sci 2004, 24:592-597
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