ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Effects Of Ginkgo Biloba Extract On Plasma Glutathion Peroxidase, Superoxide Dismutase, Adenosine Deaminase And Nitric Oxide Levels In Cisplatin-induced Nephrotoxicity
Dr. Mukaddes GÜLEÇ,a Dr. H. Ramazan YILMAZ,b Dr. Mustafa IRAZ,c Dr. Seda AĞLAMIŞ,c Dr. Sadık SÖĞÜTd
aBiyokimya, cFarmakoloji AD, İnönü Üniversitesi Tıp Fakültesi, MALATYAbTıbbi Biyoloji ve Genetik AD, Süleyman Demirel Üniversitesi Tıp Fakültesi, ISPARTAdBiyokimya AD, Mustafa Kemal Üniversitesi Tıp Fakültesi, HATAY
Objective: Cisplatin (CDDP), derived from platinium, is a broad-spectrum antineoplastic agent. It has not commonly been used as a therapeutic agent because of its nephrotoxicity risk. The underlying mechanism in nephrotoxi-city has been attributed to reactive oxygen species (ROS). Gingko biloba
extract (GBE, Egb 761) has been shown to be effective on some organ and tissue pathologies induced by ROS. The aim of this experimental study was to determine whether antioxidant GBE has a preventive effect on nephrotoxi-city induced by CDDP through oxidative damage.
Material and Methods: Male Sprague Dawley rats (60 days old) were used in the experiments. Rats were randomly assigned to one of four groups: control or untreated rats (n=7); rats treated with i.p. injection in a single dose of 7 mg/kg body wt CDDP (Cisplatin, Ebewe) (n=8); rats treated with CDDP plus i.p. injection of 10 mg/kg body wt vitamin E (Evigen-Aksu, Turkey) (n=9); and rats treated with CDDP plus oral administration of GBE in a dosage of 100 mg/kg body wt (n=7). After 10 days of experimental procedure, animals were euthanized by bleeding, kidneys were removed and oxidant and antioxidant parameters were examined to determine the effects of agents as applied to the rats.
Results: In treated rats, the activity of plasma glutathion peroxidase (GSH-Px) was decreased, but adenosine deaminase (ADA) and nitric oxide (NO) levels were increased with CDDP. The activity of superoxide dismutase (SOD) remained unchanged in all study groups. Upon treating the rats with CDDP + vit E, the activities of GSH-Px and ADA, and the level of NO were improved. In the case of CDDP + GBE application, we determined an increased activity of GSH-Px in comparison with the rats treated with CDDP (p<0.014). GBE also decreased the levels of ADA and NO, which were increased in the CDDP-treated group.
Conclusion: In keeping with the results, the main underlying mechanism in CDDP-induced nephrotoxicity appears to be due to renal tubular cell damage. GBE appeared to induce a beneficial therapeutic effect in this study. However, we can not yet consider GBE to be a new therapeutic agent in CDDP nephrotoxicity until further studies with various doses, different time intervals, and more animal numbers have been provided.Keywords: Cisplatin nephrotoxicity, ginkgo biloba, glutathione peroxidase, superoxide dismutase, adenosine deaminase, nitric oxideTurkiye Klinikleri J Med Sci 2004, 24:585-591
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