ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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Plasma Lipid Peroxidation Related Withwhole-body Gamma-irradiation In Rats
Serdar YARDIMCI*, M Cem KOÇKAR**, Neşe KÖKSAL***,
Mustafa CENGİZ****, Tuncay DELİBAŞI**, Sema YAVUZER*****
*Doç.Dr.Ankara Üniversitesi Tıp Fakültesi, Fizyoloji ABD,
**Dr.Ankara Numune Hastanesi, Endokrinoloji ve Metabolizma Hastalıkları Kliniği,
***Dr.Ankara Üniversitesi Tıp Fakültesi, Fizyoloji ABD,
****Dr.Hacettepe Üniversitesi Tıp Fakültesi, Radyasyon Onkolojisi ABD,
*****Prof.Dr.Ankara Üniversitesi Tıp Fakültesi, Fizyoloji ABD, ANKARA
In this study, the effects of whole body irradiation on plasma lipid peroxidation of rats were investigated. To examine the effects of irradiation on the level of lipid peroxidation products, male Wistar rats in experimental group (n:12) were exposed to 5-Gy of single dose external gamma-radiation. Blood samples (2 cc/100 g body weight) were taken by intracardiac puncture before the exposures. Plasma lipid peroxidation was evaluated by the measurement of malondialdehyde (MDA) levels. Concentrations of plasma MDA were assayed by the method described by Yoshioka et al. Sham-exposed rats (n:12) were subjected to the identical manipulation procedure without irradiation. At the 10th day after the exposures, the measurement were repeated in both groups. In the experimental group, means level of plasma MDA were found to be 3.5±1.7 nmol/ml and 30.9±7.2 nmol/ml pre- and postirradiation respectively. Initial and latter values of sham-exposed rats were 3.3±1.6 nmol/ml and 8.5±1.8 nmol/ml respectively. The differences between the initial and latter levels of both groups were statistically significant, but 10th day levels of irradiated rats were significantly higher than the latter values of sham-exposed rats. The increased MDA levels obtained from sham-exposed rats might be due to hypoxia induced by acute blood loss and/or to anesthesia. Our results demonstrated that single dose whole-body gamma-irradiation caused lipid peroxidation. It was considered that increased plasma lipid peroxidation might be an indicator of high doses of irradiation-induced deleterious effects in organisms.
Keywords: Gamma-radiation, Radiation injury,
Radiotherapy, Lipid peroxidation,
Malondialdehyde
Turkiye Klinikleri J Med Sci 1996, 16:434-436
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