ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Catalase-peroxidase Gene (kat G) Deletion In Isoniazid Resistant Strains Of Mycobacterium Tuberculosis
Figen ATALAY, MD,a Nejat AKAR, MD,b Dilek ERNAM TURGUT, MD,a Derya AYSEV, MD,c
Pınar ERGÜN, MD,a Yurdanur ERDOĞAN, MD,a
aAtatürk Chest Disseases and Chest Surgery Center,
bDepartment of Pediatric Molecular Pathology,
cDepartment of Microbiology, Ankara University School of Medicine, ANKARA
Objective: A recent study showed that some isoniazid-resistant Mycobacterium tuberculosis strains have a complete deletion of the gene (Kat G) encoding the catalase-peroxidase enzyme. We wished to examine what proportion of clinical isolates of isoniazid-resistant M. tuberculosis exhibit Kat G deletion in our Country.
Material and Methods: The relation between Kat G deletion and isoniazid-resistance was examined in 10 isoniazid-resistant and 26 isoniazid-sensitive, for a total of 36 isolates, using polymerase chain reaction (PCR) technique.
Results: Kat G deletion was observed in 70% of isoniazid-resistant and 7.7% isoniazid-sensitive isolates (p<0.05, x²). The Kat G deletion ratio in isoniazid-resistant isolates were observed to be higher than in isoniazid-sensitive isolates [odds ratio, 28, (95% CI, 3.87 to 202.26)]. The specificity and sensitivity of the PCR technique, which revealed both Kat G deletion and isoniazid-resistance, were 92.3% and 70% , respectively.
Conclusion: The deletion of the Kat G gene may play an important role in resistance to isoniazid in most M. tuberculosis isolates in our Country.Keywords: Catalase-Peroxidase Gene (KatG), isoniazid resistance, MDR-TB, mycobacterium tuberculosisTurkiye Klinikleri J Med Sci 2004, 24:243-246
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