ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
Structural Changes Induced By Cadmium Chloride And Effects Of Metallothionein On These Changes In Rat Testicular Tissue: A Light Microscopic Study
Dr.Neriman ÇOLAKOĞLU,a Dr.Aysel KÜKNER,a Dr.Haki KARA,b Dr.Enver OZANa
aHistoloji&Embriyoloji AD, Fırat Üniversitesi Tıp Fakültesi,
bFırat Üniversitesi, Sağlık Yüksek Okulu, ELAZIĞ
Objective: This study was undertaken to investigate cadmium chloride-induced changes and the purported protective effects of co-administration of metallothionein in the testicular tissue.
Material and Methods: Thirty-six adult Wistar rats were divided into three groups. Cadmium chloride (3.5mg/kg/day) was subcutaneously (sc) injected to the first group (n = 16), whereas the second group of rats (n = 16) received both cadmium chloride (3.5mg/kg/day sc) and metallothionein (30µmol/kg/day intraperitoneally). The 3rd group (n = 4) functioned as control. On the 1st, 3rd, 5th and 7th day of the study, testicular tissue samples were taken under ether anesthesia and fixed with 10% formaldehyde solution, then prepared in paraffin blocks for light microscopic examination. Tissue sections were stained with hematoxylen-eosin and Masson’s Trichrom. Scoring was done for histo-pathological alterations.
Results: In the macroscopic examination, all testes tissues appeared to be purplish. On light microscopic examination, particularly after the 3rd day of injection of cadmium chloride, severe tissue damage was evident. The damage gradually increased relative to the time of administration of cadmium chloride. It was determined that cadmium chloride led to thickening of the tunica albuginea, damage to seminiferous tubules and increase in interstitial connective tissue. Similar structural changes were observed in the group that was co-administered metallothionein for protection.
Conclusion: It was established that cadmium was toxic for testes and caused severe structural damage. Metallothionein, a metal-binding protein, failed to prevent cadmium chloride-induced damage.Keywords: Cadmium, metallothionein, testes, light microscopeTurkiye Klinikleri J Med Sci 2004, 24:201-206
|
|
|
|
|
