ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Relationship Between Radiation-induced Apoptosis Inrat Germ Cells And Amifostine
Dr.Ömür KARAKOYUN ÇELİK,a Dr.Arif ARAS,a Dr.Dilek TUĞAN,a Dr.Mine HEKİMGİL,a Dr.Deniz YALMAN,a Dr.Mustafa ESASSOLAK,a Dr.Ayfer HAYDAROĞLUa
aRadyasyon Onkolojisi AD, Ege Üniversitesi Tıp Fakültesi, İZMİR
Objective: Testicular tissue produces an extensive apoptotic response to irradiation. Amifostine is a chemoprotectant that is used to prevent some of the side effects of chemotherapy (cisplatin) or radiation therapy. The purpose of this study was to evaluate the efficacy of amifostine on radiation-induced apoptosis of testicular germ cells using the TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick and labelling assay) method.
Material and Methods: Thirty-four male Sprague-Dawley rats with a median weight of 250 grams were grouped into three groups, comprised of 12 rats in each of 2 study groups and 10 rats in a control group. Irradiation was applied with a Theratron 780-C Co60 teletherapy machine. A single fraction of 2 Gy was given to the whole abdomen and testes. The first group received 2 Gy radiotherapy alone, while the second group received 200 mg/m2 amifostin intraperitoneally 15 minutes prior to irradiation. The control group received neither radiation nor amifostine. The rats were sacrificed 10 weeks after irradiation for immunohistological evaluation. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick and labelling assay) was used to identify apoptosis. Apoptotic indices (AI) were obtained by evaluating ten separate seminiferous tubules on each slide randomly.
Results: A low rate of AI (1.8%) observed in the control group was evaluated as spontaneous apoptosis. Although the interval after irradiation was 10 weeks, both groups receiving 2 Gy exhibited intense apoptotic responses. The mean AI was 42% in the radiation alone group and 37% in the radiation plus amifostine group. There was no statistically significant difference between the AI’s of the study groups.
Conclusion: Intraperitoneal administration of amifostine, 15 minutes prior to irradiation, did not prevent radiation-induced germ cell apoptosis in rats.Keywords: Radiation, apoptosis, germ cellTurkiye Klinikleri J Med Sci 2004, 24:142-146
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