ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Analysis Of Dpyd Gene Using Bioinformatics Tools
Fuat DİLMEÇ, MD,a Lokman VARIŞLI,b Abdullah ÖZGÖNÜL, MD,c Osman CEN, MD,b
Ali UZUNKÖY, MDc
aDepartment of Medical Biology, Harran University, Medicine Faculty,
bDepartment of Biology, Harran University, Art and Science Faculty,
cDepartment of General Surgery, Harran University, Medicine Faculty, ŞANLIURFA
Objective: The DPYD gene, which encodes for the dihydropyrimidine dehydrogenase (DPD), is responsible for the pyrimidine catabolic pathway, and degrades more than 80% of the administered chemotherapeutic drug 5-fluorouracil (5-FU). This study aimed to investigate In silico the structure of the DPYD gene and its products.
Material and Methods: We investigated the homology, conserved domain, promoter and expression profiles of human DPYD gene in various species using bioinformatics tools, such as NCBI blast, EBI ClustalW, DigiNorthern, Mega3, and Genomatix programs.
Results: Our results revealed that DPD proteins were conserved among all organisms investigated. They had three conserved domains (GltD, DHPD_FMN, and COG1146), some of which had full and truncated sub-domains. We noted that the human DHPD_FMN and COG1146 domains were more conserved among the investigated species than the human GltD domain. With the multiple alignment strategy, protein and GltD domain sequences of Xenopus tropicalis (X. tropicalis) was predicted to have a truncation. The comparative screening of the promoters demonstrated that DPYD genes did not seem to have any common conserved transcription factor binding sites.
Conclusion: This study demonstrated that the DPD molecules in various species, except Danio rerio (D. rerio), were well conserved throughout evolution. Comparative screening of the promoter sequences of the human DPYD gene and its homologues found in the NCBI database revealed that there was no any common transcription factor binding sites.Keywords: Dihydrouracil dehydrogenase (NADP),genomics, evolution, promoter regions (Genetics), gene expressionTurkiye Klinikleri J Med Sci 2007, 27:829-837
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