ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Different Doses Of Radiation On Agar Colony Forming Development In C6 Glioma Cells: Assessment By Thymidine Labeling Index, And Bromodeoxyuridine Labeling Index
Tolga ÖZMEN, MD,a Gülperi ÖKTEM, MD,b Sevilcan TUNA,c Burcu BİLTEKİN,d Seçnur DENİR,e Ayhan BİLİR, MDc
aİstanbul University, İstanbul Faculty of Medicine, İSTANBULbDepartment of Histology and Embryology, Ege University School of Medicine, İZMİRcDepartment of Histology and Embryology, İstanbul University School of Medicine, İSTANBULdDepartment of Histology and Embryology, Edirne University School of Medicine, EDİRNEeDepartment of Biological Science, Balıkesir University Science Faculty, BALIKESİR
Objective: Gliomas are relatively frequent in adults, and are among the most malignant primary brain tumors. Glioblastoma multiforme, like many other tumors that exhibit radiation sensitivity in vitro, seems to be very resistant to radiation in vivo, thus suggesting that irradiation may not be a rate-limiting factor for malignant glioma tumor growth. In this study, we aimed to determine the optimal dose of radiation in C6 glioma colony forming assay, which is a valuable tool for antitumor treatment screening.
Material and Methods: 105 cell/lamella colony forming cells were radiated with 200 cGy, 400 cGy, 800 cGy and 1600 cGy for 10 minutes. Radiosensitiv-ity was measured systematically 24, 48, 72 and 96 hours after the radia-tion by three methods: soft-agar bilayer assay, thymidinE incorporation, and bromodeoxyuridine (BrdU) incorporation.
Results: The soft-agar bilayer assay, which assessed the colony-forming units, showed that the number of colonies in the control group (609, 3 ± 86.8) were decreased after 200 cGy (8.3 ± 3.6) and 400 cGy (7.2 ± 4.3). No colony was detected in 800 cGy and 1600 cGy irradiated cells [3H] Thymidine incorporation was more prominent with higher doses of radiation. BrdU incorporation revealed that even at low doses (200 cGy) of radiation there was a significant decrease of cell proliferation. On higher doses like 1600 cGy it was more prominent.
Conclusion: Cell survival, doubling time, and cell phases are parameters of growth kinetics, and the results suggest that C6 glioma cells are radio-sensitive and are virtually affected by all radiation doses in our experi-ment even 200 cGy at 24 hours. Besides, colony forming assay with thymidine labeling index, and BrdU labeling index may be used as new methods for determining radiotherapy doses in clinical applications.Keywords: Dose fractionation; gliomaTurkiye Klinikleri J Med Sci 2007, 27:321-327
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