ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Metabolic Stress And The Contractility Of Isolated Rat Myometrium: Role Of Potassium Channels And Different Cellular Ph Buffers?
Dr. İhsan SERHATLIOĞLU,a Dr. Mete ÖZCAN,a Dr. Selim KUTLU,b Dr. Ahmet AYARb
aBiyofizik AD, bFizyoloji AD, Fırat Üniversitesi Tıp Fakültesi, ELAZIĞ
Objective: The aim of this study was to investigate the role of different pH buffers, cellular energy sources and role of potassium channels in potassium cyanide (KCN)-induced metabolic stress- mediated inhibition of contractility of isolated rat myometrium.
Material and Methods: Myometrial strips were isolated from virgin adult Wistar rats after cervical dislocation and suspended in an isolated tissue bath containing Krebs solution, continuously bubbled with 95% O2 and 5% CO2, maintained at 370C (pH 7.4). After the development of spontaneous contractions following equilibrium under resting tension, KCN, pyruvate, glybenclamide or tetra ethyl ammonium (TEA) was added to the tissue bath. The amplitude and frequency of contractions were evaluated by 10-min intervals. Wilcoxon rank test was used for statistical analysis of data.
Results: KCN (0.5 mM) gradually inhibited the spontaneous contractions, being more effective in experiments where NaHCO3 was used in the Krebs solution instead of HEPES. This inhibition was reversed by pyruvate (10 mM), but application of ATP-sensitive K+ channel blocker, glybenclamide (100 mM), did not reverse the KCN-induced inhibition of myometrial contractions (n= 7). Furthermore, the inhibition of spontaneous myometrial contractions by KCN (0.5 mM, n=6) was reversed by the application of K+ channel blocker TEA (1 mM, n= 6).
Conclusion: Results from this study indicate that K+ channels play a role, at least partially, in KCN-induced inhibition of myometrial contractions of rat and this inhibition may be reversed by pyruvate.Keywords: Myometrium; intermediate-conductance calcium-activated potassium channelsTurkiye Klinikleri J Med Sci 2007, 27:170-178
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