ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Protective Effect Of Aminoguanidine On Random Pattern Skin Flap Survival: An Experimental Study In Rats
Hakan AYDOĞAN, MD,a Ali GÜRLEK, MD,a Hakan PARLAKPINAR, MD,b Nilay AYDOĞAN, MD,c Ahmet ACET, MD,b
Departments of aPlastic, Reconstructive and Aesthetic Surgery, bPharmacology, cInstitute of Health Sciences, Biochemistry, İnönü University Faculty of Medicine, MALATYA
Objective: Distal flap necrosis resulting from ischemia is a serious problem, and increases the cost of treatment. Reactive oxygen radicals (ROS) play an important role in tissue injury and ischemia, and may lead to partial or complete flap necrosis. Aminoguanidine (AG), a potent antioxidant, prevents ROS formation and lipid peroxidation. Besides, AG inhibits inducible nitric oxide synthase (iNOS) leading to decreased generation of nitric oxide (NO).
Material and Methods: Rats were randomly divided into three groups: Control, flap elevated saline group, and AG treated group. A caudally based rectangular flap, 3 x 10-cm was elevated on the back of the rats. Flap viability was evaluated 7 days after the initial operation, measuring necrotic areas and total flap areas by computer-assisted planimetry. Malondialdehyde (MDA), NO, glutathione (GSH), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) levels were measured in flap skin tissue to observe the effects of AG.
Results: Rate of flap necrosis and MDA, NO levels were higher in the saline group compared to the control group, while GSH, GSH-Px, and SOD enzyme activities were reduced. AG administration reduced lipid peroxidation, NO generation and increased GSH, GSH-Px, SOD enzyme activities. Furthermore, it significantly reduced the rate of flap necrosis when compared with the saline group.
Conclusion: We believe that AG, a potent antioxidant and iNOS inhibitor, has beneficial effects to improve skin flap viability when distal flap necrosis is a potential complication in longer flaps.Keywords: Aminoguanidine; surgical flaps; reactive oxygen species; malondialdehydeTurkiye Klinikleri J Med Sci 2007, 27:36-43
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