ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
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Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Improving Effects Of Aminoguanidine On The Histologic Alterations In Rat Kidneys In Diabetes
Dr. Nigar VARDI,a Dr. Mustafa IRAZ,b Mehmet GÜL,a
Dr. Feral ÖZTÜRK,a Dr. Muharrem UÇAR,c Dr. Ali OTLUb
aHistoloji Embriyoloji AD, bFarmakoloji AD, İnönü Üniversitesi Tıp Fakültesi, MALATYA
cErzurum Devlet Hastanesi, ERZURUM
Objective: This study was designed to investigate the improving effects of aminoguanidine on renal histological alterations in a streptozotocin (STZ)- induced diabetic rat model.
Material and Methods: Fifteen Sprague-Dawley adult female rats were divided into three groups: control, diabetic (D) and diabetic treatment with aminoguanidine (DAG) groups. Experimental diabetes was induced by a single intraperitoneal dose of STZ (45 mg/kg). In the DAG group, AG was added in the drinking water (1 gr/L) after administration of STZ. This was maintained until the end of the study (for 8 weeks). At the end of the experiment, blood glucose levels were determined and after the routine tissue follow-up process, kidneys were embedded in paraffin. Histochemical and immunohistochemical stains were applied and the specimens were examined with light microscope.
Results: After 8 weeks, the rats in diabetes group had significantly lower body weight and significantly higher blood glucose levels than the rats of control and DAG groups. The main histological changes resulting from diabetes were detected in glomerular and tübüler basal membrane and epithelial cells (glycogen accumulation, swelling and vacuolization). We observed the improving effects of aminoguanidine treatment on rat kidney.
Conclusion: In conclusion, chronic administration of aminoguanidine reduced renal injury in STZ- induced diabetic rats. Therefore, we believe that aminoguanidine may be used to prevent development of diabetic renal damage. However, further studies are needed to elucidate the mechanisms of the improving effect of aminoguanidine on diabetic complications.Keywords: Diabetes mellitus, experimental, pimagedine, kidney, histologyTurkiye Klinikleri J Med Sci 2006, 26:599-606
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