ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
The In Vivo Effect Of Granulocyte Macrophage-colony Stimulating Factor On Kupffer Cell Function After Partial Hepatectomy
Dr. Celal HATİBOĞLU, aDr. Ahmet ALYANAK, aDr. Bahadır ÇETİN, aDr. Sebahattin ASLAN, aDr. Abdullah ÇETİNa
a1. Genel Cerrahi Kliniği, Ankara Onkoloji Hastanesi, ANKARA
Objective: The purpose of this experimental study was to investigate the in vivo effect of granulocyte macrophage-colony stimulating factor (GM-CSF) on hepatic Kupffer cell phagocytic function by means of Kupffer cell clearance capacity (KCCC) determined by isolated hepatic in situ perfusion technique.
Material and Methods: Forty male Wistar rats were divided into 4 groups: 1- Control n= 8, 2- CSF-control n= 8, 3- Experiment n= 12, 4- CSF-experiment n= 12. Controls and CSF-controls (groups 1-2) underwent laparatomy (Sham operation) and 70% partial hepatectomy was performed on the subjects of experimental groups 3-4. In the postoperative period, groups 1 and 3 received daily subcutaneous injections of saline whereas groups 2 and 4 were administered subcutaneous 0.1 mg/day GM-CSF (LeucomaxR). Four rats died-2 in group 3 and another 2 in group 4-and were excluded from the study. After 7 days all subjects underwent re-laparatomy and isolated hepatic in situ perfusion. Perfusate introduced through the portal vein contained fluorescein-labeled latex spheres of 1 mm that could only be removed by phagocytosis. KCCC expressed as percentage was extrapolated from the difference in fluorescence between influent and effluent perfusates.
Findings: The mean KCCC was 35.78 ± 6.56% in the control group and 38.45 ± 5.15% in the CSF-control group; this difference was not statistically significant. KCCC was 25.76 ± 5.10% in the experiment group and 33.07 ± 6.38% in the CSF-experiment group. The difference between experimental groups was significant.
Results: It was concluded that the administration of GM-CSF to partially hepatictomized rats increased in vivo phagocytic function of Kupffer cells but no such effect was observed in rats with normal liver.Keywords: Liver, liver regeneration, Kupffer cells, Granulocyte-Macrophage Colony-Stimulating FactorTurkiye Klinikleri J Med Sci 2006, 26:56-61
|
|
|
|
|
