ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
Histological Evaluation Of Liver And Spleen To Determine The Efficacy Of Caspofungin And Combination Of Caspofungin With Mer Penem In Candida Albicans Infected Mice
Dr. Serdar FİLİZ,a Dr. Hakkı DALÇIK,a Dr. Pelin C. BIYIKSIZ,a Dr. Sema Özcan KEÇELİ,b Dr. Fatma BUDAKb
aHistoloji ve Embriyoloji AD, bMikrobiyoloji AD, Kocaeli Üniversitesi Tıp Fakültesi, KOCAELİ
Objective: Candida albicans is a major cause of morbidity and mortality in patients with compromised immune function. The aim of this study was to determine the histological changes in the liver and spleen in murine systemic candidiasis and to evaluate histologicaly the efficacy of caspofungin and the combination of caspofungin plus meropenem in the treatment.
Material and Methods: BALB/c mice were infected intravenously with 2 x 106 cfu of C. albicans. At 24 hours postinfection, intraperitoneal therapy was initiated and continued for 7 days. Therapy groups included those given caspofungin 0.5 mg/kg, 1.25 mg/kg, or 5 mg/kg/day (once daily) and meropenem 20 mg/kg/day (twice daily) in combination with caspofungin. On day 8, livers and spleens were removed and after the routine processes, histological changes were examined.
Results: Lymphocytic infiltration around the vessels and cystic channels due to C. albicans infection were present at the portal areas of the liver preparations. This infiltration decreased with high dose caspofungin. In the combined treatment group, liver destruction increased parallel to the increasing caspofungin dose. Generalized fibrosis, relative decrease of and irregularity in the white pulp, and large number of giant cells due to C. albicans infection were noted in spleen preparations. While histological improvement was restricted in caspofungin groups, fibrosis was lacking despite the presence of giant cells in the combination groups.
Conclusion: In systemic candidiasis, high dose caspofungin and meropenem combination therapy was effective in acute splenitis but caused serious liver destruction.Keywords: Candida albicans, liver, spleen, caspofungin, meropenemTurkiye Klinikleri J Med Sci 2005, 25:763-769
|
|
|
|
|
