ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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Unbiased Stereological Methods For Number Estimations Of The Objects In Tissue Specimens: The Physical Disector
Bünyami ÜNAL*, Sinan CANAN**, Hüseyin ASLAN***, Bünyamin ŞAHİN****,Osman ÇATALOLUK*****, Süleyman KAPLAN******
* Öğr.Gör.Dr., Gaziantep Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD, GAZİANTEP** Arş.Gör.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Fizyoloji AD,*** Uz.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Histoloji- Embriyoloji AD,**** Yrd.Doç.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Anatomi AD, SAMSUN***** Öğr.Gör.Dr., Gaziantep Üniversitesi Tıp Fakültesi Tıbbi Biyoloji AD, GAZİANTEP****** Prof.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD, SAMSUN
The disector method relies upon the estimation of the number of objects within a three dimensional volume consists of a pair of serial sections that are separated by a known distance, "t". This method was first described in a paper by Cruz-Orive (1) and this paper is considered to be the first modern design-based stereological paper. The method of serial sectioning without reconstruction as an unbiased technique for number estimation was also illustrated practically by De Groot and Bierman (2). However even without reconstruction, exhaustive serial sectioning was extremely tedious and has proven too labour intensive to be used routinely. Serial sectioning without reconstruction represented a real unbiased and assumption-free approach to counting objects in three dimensional volume. However the major breakthrough in stereological counting was provided by the publication of "the disector" by Sterio (1984). This method enables the researcher to determine the number of any object in a given volume independent from the size, shape, orientation of object, the thickness of the sections and shrinkage of the tissues. All these features make this method an unbiased and efficient way of counting the number of biological particles in tissue specimens.
Keywords: Stereology, Disector
Turkiye Klinikleri Tıp Bilimleri 2002, 22 (S):15-24
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