ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Effects Of Caffeic Acid Phenethyl Ester Ondna-turnover Rates And Nitric Oxide Level In Doxorubicin-induced Myocardial Injury
Ersin FADILLIOĞLU*, Hasan ERDOĞAN**, Sadık SÖGÜT***, İrfan KUKU****
* MD, Asis.Prof., Dept. of Physiology, Medical School of Inonu University,** MD, Dept. of Physiology, Medical School of Inonu University, MALATYA*** MD, PhD, Asis.Prof., Dept of Biochemistry, Medical School of Mustafa Kemal University, HATAY****MD, Asis.Prof., Dept. of Hematology, Medical School of Inonu University, MALATYA, TURKEY
Purpose: Doxorubicin (DXR), an important chemotherapeutic agent for cancers, has severe cardiotoxic effects. This work was designed to determine whether the doxorubicin-induced cardiotoxity via changes in purine catabolism, nitric oxide (NO) system and collagen formation and is prevented by caffeic acid phenethyl ester (CAPE).
Materials and Methods: Male Sprague-Dawley rats (60 days old) were divided into three groups. One group was untreated and the others were treated with DXR or DXR+CAPE, respectively. DXR was administered by a single i.p. injection (20 mg/kg). CAPE was administered i.p. 10 µmol/kg/day two days before DXR treatment for 12 days. Hydroxyproline (OH-P) formation was determined in myocardium. The changes in purine catabolism and NO system were determined by the activities of xantine oxidase (XO) and adenosine deaminase (ADA) and NO level in the heart tissue, respectively.
Results: DXR treatment without CAPE increased OH-P level significantly in myocardial tissue. The rats treated with CAPE produced significant decrease in OH-P level in comparison with DXR group. The activities of XO and ADA were significantly higher in DXR-treated rats in comparison with control and DXR+CAPE-treated rats. DXR treatment increased tissue NO level in myocardium and CAPE prevented this increase. There was no significant difference in NO levels between control and DXR plus CAPE-treated rats.
Conclusion: The protection of heart tissue by CAPE againts DXR-induced myocardial injury was demonstrated by decreased
OH-P level upon CAPE administration to the rats. Increased XO and ADA enzyme activities may indicate high DNA turn over rates in heart tissue due to DXR-toxicity. CAPE may prevent DXR-induced increased DNA turn over rates and preserve myocardium from injury. İncreased NO level, as a free radical, may be regarded as an index of myocardial damage due to DXR, Furthermore, CAPE inhibited excessive NO production and prevented pro-inflammatory effects of NO and so might protect tissue from injury due to high inflammatory reaction as indicated previously in the literature.Keywords: Doxorubicin, Caffeic Acids, Hydroxyproline, Nitric oxideTurkiye Klinikleri J Med Sci 2003, 23:366-370
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