ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Effects Of One Dose And Seven Days’ Managements Of Melatonin And Steroid To Prevent Ischemia-reperfusion Injury In Testicular Torsion
Dr. Müslim YURTÇU,a Dr. Adnan ABASIYANIK,aDr. Mustafa Cihat AVUNDUK,b Dr. Emrah KARAGÖZOĞLU,c Dr. Fatma ABASIYANIKd
aÇocuk Cerrahisi AD, bPatoloji AD, Selçuk Üniversitesi Meram Tıp Fakültesi,
cBiyokimya Bölümü, dPatoloji Bölümü, Konya Numune Hastanesi, KONYA
Objective: The aim of the study is to determine the effects of melatonin and steroid treatments which were injected as one-dose and one-dose for seven days to prevent ischemia-reperfusion injury occurred in testicle after torsion and detorsion and which of these treatments is more effective.
Material and Methods: Eighty prepubertal male Sprague-Dawley rats were devided into 8 groups each containing 10 rats. In all groups, except sham groups, torsion was created by rotating the left testicles 720o in a clockwise direction for 6 hours. In melatonin groups 17 mg/kg melatonin and in steroid groups 1 mg/kg methylprednesolone were injected before 30 minutes from detorsion. In seven dose steroid and melatonin groups melatonin and methylprednesolone were injected once a day for 7 days. In Sham-1, torsion-detorsion-1, one-dose melatonin and, one-dose steroid groups left orchiectomies were created after 6 hours of detorsion. In other group, left orchiectomies were performed 7 days later. After performing histopathologic examination we determined MDA levels and Johnsen’s scores of testicular tissue to identify ischemia-reperfusion injury.
Results: MDA levels in one-dose melatonin and steroid groups were significantly less compared with torsion-detorsion-1 group (p< 0.05). Johnsen’s scores were the same as in one-dose melatonin, steroid and torsion-detorsion-1 groups (p> 0.05). It was identified that MDA levels in seven dose melatonin and seven dose steroid groups were significantly less compared with torsion-detorsion-2 group (p< 0.01) and Johnsen’s scores were significantly higher compared with torsion-detorsion-2 group (p< 0.01).
Conclusion: The treatments of one-dose melatonin and steroid are effective against the I-R damage biochemically, but not histopathologically. Whereas, melatonin and steroid, which were injected once a day for 7 days, prevent ischemia-reperfusion injury completelyKeywords: Spermatic cord torsion, reperfusion injury, melatonin, steroidsTurkiye Klinikleri J Med Sci 2005, 25:496-500
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