ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
Effect Of Doxorubicin On A Mcf-7 Cell Line Multicellular Tumor Spheroid Model: An Electronmicroscopic Study
Dr. Gülperi ÖKTEM,a Dr. Şule AYLA,b Dr. Sevilcan TUNA,c Dr. Meral BAKA,a Dr. Ayhan BİLİRc
aHistoloji ve Embriyoloji AD, Ege Üniversitesi Tıp Fakültesi, İZMİRbHistoloji ve Embriyoloji AD, İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi,cHistoloji ve Embriyoloji AD, İstanbul Üniversitesi İstanbul Tıp Fakültesi, İSTANBUL
Objective: Many properties of human tumor cells that propagate in three-dimensional multicellular spheroids (e.g., drug sensitivity, junctional complexes, and invasive capabilities) differ from those exhibited in monolayer cell cultures. Multicellular spheroids may offer convenient in vitro experimental models capable of occupying a niche between monolayer cell cultures and in vivo tumors. In the present study, the effects of doxorubicin on human breast cancer cells were investigated ultrastructurally with the aid of spheroids.
Material and Methods: We employed the liquid overlay technique of obtaining spheroids. MCF-7 breast cancer cell spheroids were then electron-microscopically examined for the effects of subsequently applied doxorubicin.
Results: Tumor cells exhibiting evidence of the apoptotic process were seen in spheroid centers.
Conclusion: In this study, in addition to annotating the physiological and pathological changes manifested in the formation of tumors, many of the effects of chemotherapy on tumor cells were elucidated. The spheroid method was determined to be useful in the assessment of treatment efficacy with various chemotherapeutic agents.Keywords: MCF-7 (estrogen receptor related protein p29, human), doxorubicin, microscopy, electron, neoplasmsTurkiye Klinikleri J Med Sci 2005, 25:337-342
|
|
|
|
|
