ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
A Pre-study About Hepatic Malondialdehyde, Nitric Oxide Levelsand Superoxide Dismutase, Glutathione Peroxidase Enzymeactivities In Mice With Candidiasis
Neslihan BUKAN*, Banu SANCAK**, Ayşe KALKANCI***, Semra KUŞTİMUR****
* Uz.Dr., Gazi Üniversitesi Tıp Fakültesi Biyokimya AD,
** Yrd.Doç.Dr., Gazi Üniversitesi Tıp Fakültesi Biyokimya AD,
*** Öğr.Gör.Dr., Gazi Üniversitesi Tıp Fakültesi Mikrobiyoloji AD,
****Prof.Dr., Gazi Üniversitesi Tıp Fakültesi Mikrobiyoloji AD, ANKARA
Purpose: Reactive oxygen species have been increasingly implicated as playing a central role in the pathophysiology of clinical infections. In this study, in order to evaluate the role of NO and antioxidant enzyme activities in mice with candidiasis, we have measured hepatic malondialdehyde (MDA), nitrate+nitrite levels and glutathione peroxidase (GSH-Px), superoxide dismu-tase (SOD) enzyme activities.
Materials and Methods: 6-8 week, female, BALB/c twenty mouse were enrolled in this study. Ten mouse were used as control group and 10 mouse were infected by C. albicans. Animals were sacrificed with high dose ketamine hydrochloride (KetalarÒ, Eczacıbaşı, Turkiye). The tissues were briefly washed in ice-cold 0.9% saline (w/v), then frozen in liquid nitrogen and were stored at -70 °C until the subsequent protein and enzyme assays. Malondialdehyde (MDA), nitrite + nitrate levels and glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) enzyme activities were determined in liver tissues.
Results: Hepatic MDA and nitrate+nitrite level in rats with candidiasis were found significantly higher than the con-trols (p<0.05, p<0.001, respectively). On the other hand, hepatic GSH-Px activity was significantly lower in rats with candidiasis (p<0.01) whereas SOD activity didn’t change in liver.
Conclusion: Hepatic injury can be generated by C. albicans infection and during this process antioxidant defense mechanism is not enough to protect the organism.Keywords: Candida albicans, Malondialdehyde, Nitric oxide, Superoxide dismutase, AntioxidantsTurkiye Klinikleri J Med Sci 2003, 23:285-288
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