ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Histological Changes Caused By Long Term Alcohol Consumption On Rat Pancreas
Nigar VARDI*, Feral ÖZTÜRK**
* Öğr.Grv.Dr., İnonü Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD,
** Yrd.Doç.Dr., İnonü Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD, MALATYA
Several studies have confirmed the close association between alcohol consumption and pancreatic damage. Although chronic ethanol consumption causes pancreatic disease, little is known about its effect on pancreas histology. The present experiment was planned to study the effects of alcohol on the pancreas histology of Wistar albino rats at the light and electromicroscopic levels. The study was performed on 17 male Wistar albino rats. The laboratory animals were divided into two groups as experiment and control. The experimental group was fed with a modified liquid diet (MLD) containing 7.2 % of ethanol during six months. Control rats were fed by isocaloric MLD without ethanol. At the end of the experiment rats were sacrified by servical dislocation and their pancreas tissue were excised. Samples were fixed in Bouin’s solution for light microscopy; in fosfate buffered 2% OsO4 for EM. After routine tissue process for light microscopy, tissue sections were embedded in paraffin. 6m section were stained with H&E and Masson’s trichrome methods. For EM examination, tissue section were dehydrated with ethyl alcohol series, thick and thin sections were embeded in Araldite. Semithin sections were examined by light microscope and ultrathin sections examined by electron microscope ultrastructurally.The results are as follows: 1) Anisocytosis and dissarrangement of aciner cells were prominent. It is observed that, aciner cells lost their distinguishing characteristics and also lost zymogen granules which identify them as aciner cells. Fat droplets and myelin-like figures were frequent findings within the cytoplasma of aciner cells. They were also seen among the cysternae of granular endoplasmic retikulum in these cells. The earliest and most consistent pathological finding in the ethanol group was the interdigitation between the aciner and centroaciner cells. 2) Aciner tissue was disorganized and atrophic. In these areas there were an increasement in intercalated ductal cells. Intralobular ducts were enlarged and ductal epithelium was flattened.Keywords: Ethanol, pancreas,rats, anatomy & histologyTurkiye Klinikleri J Med Sci 2002, 22:271-275
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