ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Influence Of Smoking On Dna Damage And Blood Glutathione Level
Yıldız DİNÇER*, Eyüp İlker SAYGILI**, Tülay AKÇAY***
* Ph.D., İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Biyokimya AD,
** M.Sc. Biyolog, İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi Biyokimya AD,
*** Prof.Dr., İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, Biyokimya AD, İSTANBUL
Purpose: Smoking habit is accepted as an important risk factor for certain types of cancer. Free radicals derived from smoking play an important role in the carcinogenesis by interacting with DNA. Susceptibility to oxidative stress and cancer is determined by the status of endogenous antioxidants which are protective against the harmful effects of free radicals. The aim of the present study was to evaluate the DNA damage and status of reduced glutathione (GSH) which is a powerful endogenous antioxidant in male smokers.
Materials and Methods: 27 male smokers (smoking 20 cigarette per day) and 23 male nonsmokers were included the study. They were not suffering from any disease. Venous blood was taken into heparinised tubes. Whole blood GSH level and DNA strand breakage frequency as an index for DNA damage in leukocyte DNA were measured. A spectrophotometric method and the Comet assay (single gell cell electrophoresis) were used for the determination of GSH and DNA strand breakage frequency, respectively.
Results: Whole blood GSH level was found to be decreased (P<0.001) but DNA strand breakage frequency was found to be increased (P<0.001) in male smokers as compared to controls. A negative correlation was determined between DNA strand breakage frequency and GSH (r: -0.62, P<0.01).
Conclusion: It was thought that DNA damage is increased because of decreased GSH level in male smokers.Keywords: Smoking, DNA Damage, Comet Assay, GlutathioneTurkiye Klinikleri J Med Sci 2003, 23:108-111
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