ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Structural Changes In Second Generation At The Rats Kidney And Liver Tissues Which Have Experimentally Acquired Chronic Fluorosis
Erdal KARAÖZ*, Kanat GÜLLE**, Ethem F. MUMCU***, Alpaslan GÖKÇİMEN****, Meral ÖNCÜ****
* Prof.Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD,
** Araş.Gör.Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD,
*** Prof.Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi, Ortopedi ve Travmatoloji AD,
**** Yrd.Doç.Dr. Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD, ISPARTA
Purpose: Fluoride is considered as one of the potent anions to which man is exposed either due to ingestion or to inhalation. In either case, fluoride interacts closely with body tissues to produce chronic disease states primarily referable to the dental skeletal systems, when consumed in excess quantitie. Materials and Methods: In this study, at the start of the experiment, 24 (Wistar albino) rats in 6 groups, each with 1 male and 3 females were animal subject. The rats of each group were housed in a cage for fertilization during a night. In the following day, the females of each group were examined by the vaginal pulpation to identify whether they are pregnant or not. All the pregnant rats of (n=12) 12 were divided into 3 groups. Group 1 received commercial spring water which include 0.07 ppm, Group 2 received 100 ppm, while Group 3 received 150 ppm, fluoride in the same spring water in the drinking water during the gestation periods. The rats had labour at the 21 day, and the mother rats were kept to receive the fluoridated water at the same levels during the lactation period. Of the adult rats of first generation, were chosen to kept in 6 groups of cages and subjected to the same experimental procedure. The experiment was lasted with the same number of rats in each group until obtaining the second generation rats in the respective groups. All the mature rats of second generation who received the fluoridated water during all the growing periods until they get mature were killed at the end of 3 months. These rats were albino adult male rats of wistar strain weighing from 180 to 200g. Then, all the rats were cut and tissue samples were taken from kidney. Tissue samples were fixed in a buffered formaline, processed through graded of alcohols and xylene, and embedded in paraffin blocks. Tissue sections of 4-6mm were made at multiple levels. Sections were routinely stained with heamatoxylin-eosine. Mouted slides were examined and photographed under a light microscope.Results: Histopathological examination kidney and liver sections showed clear differences between controls and experimental animals. Renal histological changes consisted of degenerative changes in renal tubule cells, irregularly shaped nuclei with marginated chromatin. On the other hand, we have observed fibrosis in the portal area of liver tissue and proliferation in bile ducts, abnormal focal cellular infiltration in the periphery of lobe, dilatation in sinusoidal capillaries and hetochromatisme in the nuclei of hepatocytes which occurred much more severe proportional to higher dose. Conclusions: Results form related animal experiments previously performed in our study indicate that long term fluroid treatment at these doses can induce a series of functional changes, metabolism disorders and histopathological changes in rats kidney and liver tissues.Keywords: Fluorides, Drug Toxicity, Liver, Kidney, RatsTurkiye Klinikleri J Med Sci 2003, 23:129-134
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