ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Plasma L-carnitine Levels In Non-pregnant, Pregnant And Lactating Rabbits
Abdurrahman COŞKUN*, Mahmut DOĞAN**, E. İnci KARAKÜÇÜK***
* Uz.Dr., Kırıkkale Devlet Hastanesi Biyokimya Bölümü, KIRIKKALE
** Yrd.Doç.Dr., Erciyes Üniversitesi Mühendislik Fakültesi Gıda Mühendisliği Bölümü,
*** Doç.Dr., Erciyes Üniversitesi Tıp Fakültesi Biyokimya AD, KAYSERİ
Purpose: Fatty acids are an important fuel source for neonates. The utilization of long chain fatty acids as a fuel source is dependant upon adequate concentrations of carnitine. Decreased tissue, blood and urine carnitine concentrations in premature infants receiving soy-based infant formulas or carnitine–free parenteral nutrition, compared to healthy infants receiving milk-based diet, provide evidence that the fetus may not be capable of substantial carnitine synthesis and receives carnitine from maternal pool. In rabbits as a model; we planned this study to examine the changes of carnitine levels in plasma, during dry, pregnancy, and lactating periods. Materials and Methods: Seventeen New Zeland rabbits were involved in this study. Serial blood samples were collected from rabbits on dry (non-pregnant) period, 10-13 and 20-23 days of gestation and after 4 days of lactation. A high–performance liquid chromatographic (HPLC) method was used to determine free carnitine in plasma. For determination of total carnitine, plasma samples were hydrolyzed with KOH prior to carnitine assay. The difference between total and free carnitine was considered to be acylcarnitine. Results: Plasma free acyl and total carnitine concentrations during the 1st period (10–13.days) of gestation did not change significantly, compared with non-pregnant (dry) period (p>0.05). During the second period of gestation (20-23rd days), a significant decrease of free and total carnitine concentrations were observed (p<0.01), but the decrease of acylcarnitine concentration was not statistically significant (p>0.05). In opposite to this situation, during lactation period plasma free, total and especially acylcarnitine concentrations increased significantly (p<0.001), compared with dry and pregnancy periods.Conclusion: We concluded that, increasing plasma volume and carnitine transfer to fetus during pregnancy period, and hormonal changes during pregnancy and lactation periods, may affect plasma carnitine levels in pregnant and lactating rabbits.Keywords: Carnitine, Pregnancy, Fatty Acids, Chromatography, High Pressure LiquidTurkiye Klinikleri J Med Sci 2003, 23:5-9
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