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Errors Due To Use Of The Numerical Density In The Sections Inexperimental Studies
Bünyamin ŞAHİN*, Hüseyin ASLAN**, Bünyami ÜNAL***, Sinan CANAN****,
Serdar ÇOLAKOĞLU*****, Sait BİLGİÇ******, Süleyman KAPLAN*******
* Yrd.Doç.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Anatomi AD, SAMSUN
** Uz.Dr., Gülhane Askeri Tıp Akademisi Histoloji-Embriyoloji AD, ANKARA
*** Öğr.Gör.Dr., Gaziantep Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD, GAZİANTEP
**** Arş.Gör.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Fizyoloji AD,
***** Arş.Gör.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Anatomi AD,
****** Prof.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Anatomi AD,
******* Prof.Dr., Ondokuz Mayıs Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD, SAMSUN
The amount of the particles appeared in tissue sections, which are systematically or randomly obtained for an experimental study, is known as the numerical density of the particles. It is thought that the proportion of numerical density of the particles (i.e. glomerulus, cell, mitochondria) with the all sections in the same animal reflects the real number of the related particle. However, recently developed stereological methods proved that it is not always the case. The technical mistakes for the use of numerical density come from three natural sources; the differences of the size and the orientations of the particles under examination, the effect of section thickness and the volume changing of the tissue samples. Using stereological methods, it is possible to prevent from these mistakes and provide unbiased results.
Keywords: Numerical density, Experimental studies, Section thickness, Volumetric changes, Stereology
Turkiye Klinikleri J Med Sci 2002, 22 (S):25-29
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