ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
Plasma Total Thiol And Erythrocyte Reduced Glutathione Levels In Patients With Type Ii Diabetes Mellitus
A. Erol YAZICI*, Hatice PAŞAOĞLU*, Doğan YÜCEL*, Nermin ÇELEBİ*, Fatih BAKIR*, Mesut ÖZKAYA**
* Dr., S.B. Ankara Eğitim ve Araştırma Hastanesi Biyokimya Laboratuvarı,
** Dr., S.B. Ankara Eğitim ve Araştırma Hastanesi İç Hastalıkları Kliniği, ANKARA
Diabetes mellitus (DM) is a progressive disease and it may threat life in respect to its complications. Recently, free radicals, oxidative stress, antioxidants and familial susceptibility have been found associated with diabetes mellitus. The results of the studies have shown that free radicals increased in diabetic process.We aimed to investigate the status of antioxidant system in patients with Type II DM. We studied red blood cell reduced glutathione (GSH), an antioxidant rapidly affected by free radicals and plasma total sulphydryl (-SH) levels as an indicator of oxidative stress in new diagnosed diabetic patients and in patients treated with oral antidiabetic (OAD) agents. We compared the results of patient groups with those of the control.A total of 40 Type II diabetic patients (20 new diagnosed and 20 treated with OAD) were selected as patient groups. Red blood cell GSH levels were significantly decreased in both of the new diagnosed (7.55 ± 2.12 µmol/g Hb) and OAD group (7.81 ± 1.18 µmol/g Hb) as compared with control (8.77 ± 1.03 µmol/g Hb) (p<0.05 and p<0.01, respectively). Plasma -SH levels were significantly decreased in both new diagnosis (605.64 ± 36.02 µmol/L) and OAD group (604.61 ± 56.06 µmol/L) as compared with controls (664.79 ± 60.13 µmol/L) (p<0.001 and p<0.01, respectively). Plasma -SH / total protein ratios were also significantly decreased in both new diagnosed (8.24 ± 0.68 µmol/g protein) and OAD group (8.19 ± 0.97 µmol/g protein) as compared with controls (9.48 ± 1.02 µmol/g protein) (p<0.001 and p<0.001, respectively). In conclusion, in type II diabetic patiens we found that plasma -SH and red blood cell GSH levels were decreased as an indicator of impaired oxidant/antioxidant balance due to oxidative stress. The impairement of oxidant / antioxidant balance may contribute to the occurrence of the complications of diabetes in patients with Type II DM.Keywords: Diabetes Mellitus, Oxidative Stress, GlutathioneTurkiye Klinikleri J Med Sci 2002, 22:487-492
|
|
|
|
|
