ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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Biomarker Of Invivo Oxidative Dna Damage; 8-hydroxy-2’-deoxyguanosine
Beran YOKUŞ*, Dilek Ülker ÇAKIR**
* Arş.Gör., Dicle Üniversitesi Veteriner Fakültesi Biyokimya AD,** Arş.Gör., Dicle Üniversitesi Tıp Fakültesi Biyokimya AD, DİYARBAKIR
It is known that oxidative stress produces an number of lesions in DNA such as base lesions, sugar lesions, DNA-Protein cross-links, single-strand breaks, double-strand breaks and abasic sites by a variety of mechanisms. Oxidative DNA damage is implicated in mutagenesis, carsinogenesis and aging. Guanine has the lowest ionization potential in the consist of DNA for this reason it exposed of free radicals. The modified base 8-hydroxy-2'-deoxyguanosine is a sensitive marker of the DNA damage due to hydroxyl attack at the C8 of guanine. 8-hydroxy-2’-deoxyguanosine is one of the 23 oxidative base adducts resulting from reactive oxygen species induced damage to DNA. It mainly induces GC-AT transversions and therefore may be responsible for mutagenesis and carsinogenesis by showing premutagenic property. Although several diseases have been investigated in relation to the ethiological importance of 8-hydroxy-2'-deoxyguanosine, further studies should be carried to investigate the role of oxidative DNA damage in disease status. 8-hydroxy-2'-deoxyguanosine can be utilized to determine amount of the antioxidants that are necessary to take on the daily diet and to investigate effect of cancerogen materials. To understand the importance and mechanism of DNA damage it is necessary to carry out accurate and precise measurement of 8-hydroxy-2’-deoxyguanosine. For this purpose, different analytical techniques such as high pressure liquid chromatography, gas chromatography and immunochemical methods have been used for its measurement.Keywords: 8-hydroxy-2'-deoxyguanosine, Oxidative damage, Free radicalsTurkiye Klinikleri J Med Sci 2002, 22:535-543
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