ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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The Effect Of Dimethyl Sulphoxide (dmso)after Reperfusion In Liver Ischemia(an Electron Microscopical Study)
İrfan COŞKUN*, Gülara HÜSEYİN
* Doç.Dr.,Trakya Üniversitesi Tıp Fakültesi, Genel Cerrahi AD,
** Doç.Dr.,Trakya Üniversitesi Tıp Fakültesi, Patoloji AD, EDİRNE
Ischemia is one of the important causes of liver cell injury and it was known that the free oxygen radicals play very effective role in this type of injury. We examined the effect of Dimethyl Sulphoxide (DMSO) after reperfusion in liver ischemia that was produced on rat liver cells in this study.
Fourty rats were divided in two groups as experiment (group A1, A2) and control (group B1, B2) groups. After a laparatomy was performed on , a 1 gr/kg dose of 5% DMSO was administered to groups A1 and A2. In order to cause ischemia portal veins, hepatic artery and bile ducts were clamped for an hour. The animals were sacrificed either on the 2nd (groups A1 and B1) or on the 14th ( groups A2 and B2) days and livers were taken out as a whole for pathological examination. Blood samples were drawn to determine the serum levels of SGOT, SGPT, LDH, and AP.
Only SGOT values in group A1 were significantly lower than that of group B1 (p<0.05) whereas SGPT,LDH and AP values in group A2 were significantly lower than that of group B2 (p<0.05). Among the other blood parameters no significant difference was found.
The tissue samples were examined under electron microscopy. Control group revealed mostly edema in hepatocyte mitochondria; clarification of matrices with homogenization of some; fragmentation and/or loss of cristae; all with reservation of outer membrane. Endoplasmic reticulum (ER) were dilated and ribosomes decreased in number. Experimental group revealed mostly normal hepatocyte mitochondria and other organelles. Ribosomes increased in number.
It was seen that DMSO decreases the effects of ischemia in the liver.
Keywords: Liver; Ischemia, Dimethyl Sulphoxide
Turkiye Klinikleri J Med Sci 1998, 18:240-244
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