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The Effects Of Glutathione Depletion On Liver Function And Hystopathology In Endotoxemic Rats
Betül BOZKURT*, Mahmut KOÇ*, Faruk COŞKUN*, Ömer CENGİZ*, Aydın BİLGİN*,
Ediz DEMİRPENÇE**, Kamer KILINÇ**, Fehmi AKSOY***
* Dr.,Ankara Numune Eğitim ve Araştırma Hastanesi 2. Genel Cerrahi Kliniği,
** Dr.,Hacettepe Üniversitesi Tıp Fakültesi Biyokimya AD,
*** Dr.,Ankara Numune Eğitim ve Araştırma Hastanesi Patoloji Laboratuvarı, ANKARA
In this experimental study; pathological and functional disorders occured in liver are inspected at rats at which intraperitoneally endotoxemia created in deficiency of major antioxidant of glutathione. The harmfull effect of different level of glutathione deficiency with endotoxemia is observed pathologically and biochemically according to endotoxemia created rats. Biochemical observations are done in two stages. Serum SGOT, SGPT, ALP, LDH, GGT, total bilirubin and direct bilirubin between groups and individuals are statistically compared and evaluated before and after medication. Tissue reducted, oxidized, total glutathione; lactic acid, hypoxhantine and lipid peroxidation levels compared between groups utilizing one way anova, Duncan’s test, paired T test, khi square test. All pathological and biochemical observations are done by one blind method. As a result deficiency of glutathione increases the cellular injury created by endotoxemia proportional with the level of absence biochemically and pathologically. Lipid peroxidation, lactic acid and hypoxhantine levels are the indicators of cellular injury and ischemia at rats with glutathione deficiency are observed to have a statistically significant increase. The clinical importance of glutathione in endotoxemia for preventing the cellular injury created by free oxygene radicals; for the maintenance of cellular integrity and metabolism are proved by the supporting results of pathological and biochemical in this study.
Keywords: Endotoxemia, Glutathione depletion,
Free oxygene radicals
Turkiye Klinikleri J Med Sci 1998, 18:388-397
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