ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Effect Of Allopurinol And Acetylsalicylate On Ischemia-reperfusion Related Injury Of Liver
Mehmet AKÖZ*, Alaaddin DİLSİZ**, Mehmet GÜRBİLEK*, Aytekin KAYMAKÇI**,Mahmut AY*, Fatih GÜLTEKİN*
*Departments of Biochemistry, **Pediatric Surgery Faculty of Medicine, Selçuk University, Konya, Turkey
It is assumed that, the injury which occurs in tissues during ischemia period increases during reperfusion period, and this increase is due to lipid peroxidation caused by oxygen derived free radicals in biomembranes. Free radicals are formed through various mechanisms and two of them are xanthine oxidase system and arachidonic acid pathway. The aim of the present study is to evaluate the effect of these systems on free oxygen radicals production and lipid peroxidation by using allo-purinol (a xanthine oxidase inhibitor) and acetylsalicylate (an inhibitor of arachidonic acid metabolism) in the rat model of liver ischemia - reperfusion.
For that purpose, 28 Wistar albino rats were divided into five groups. No operation was performed on the rats in the control group. Ischemia was induced by clamping right upper branches of hepatic artery and portal vein for 90 minutes in the second group. In the third group, after 90 minutes of ischemia period, reperfusion was achieved by opening the clamps for 30 minutes. The same ischemia and reperfusion procedures were performed on the allopurinol pretreated rats in the fourth group and on the acetylsalicylate pretreated rats in the fifth group. Allopurinol and acetylsalicylate were administered by oral route for three days before the procedures. In all groups, lipid peroxidation was evaluated by measuring malondialdehyde-thiobarbituric acid (MDA-TBA) levels using thiobarbiturate method. It was observed that ischemia increased lipid peroxidation and this increase was enhanced during reperfusion period. In the allopurinol group, lipid peroxidation significantly diminished compared with reperfusion group (p<0.001) while no change was observed in the acetylsalicylate group.
Our findings suggest that xanthine oxidase system is more effective than arachidonic acid pathway on ischemia-reperfusion related injury of liver and, allopurinol has a protective effect on ischemia - reperfusion injury.Keywords: Ischemia, Reperfusion, Allopurinol,
Acetylsalicylate, Liver injuryTurkiye Klinikleri J Med Sci 1999, 19:95-99
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