ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Does Fetal Hepatocyte Transplantation Provide Metabolic Support Immediately After Surgically Induced Acute Hepatic Failure In Rats?
Alaeddin DİLSİZ*, Burhan KÖSEOĞLU*, Aytekin KAYMAKÇI*,
Murat AKTAN**, Selçuk DUMAN**, Ender ERDOĞAN**
Departments of *Pediatric Surgery, and **Histology Selçuk University, Faculty of Medicine, Konya, TURKEY
Acute hepatic failure (AHF) is associated with a high mortality rate. The surgical approach for patients with AHF is orthotopic liver transplantation. However, this approach involves many risks and difficulties. If proper supportive therapy is given during the critical period of failure the liver may have the opportunity to regenerate and recover by itself.
In this study, intrasplenic fetal hepatocyte (FHc) transplantation was evaluated for providing metabolic support in Wistar Albino rats with AHF produced by 90% hepatectomy. We divided the rats into four groups as follow: Group 1: Fetal hepatocytes (0.1 ml of FHc suspension: 2x106 cells) were transplanted into the spleen 4 months before AHF. Group II: Fetal hepatocytes were transplanted to the spleen at the same time of induced AHF. Group III: Hank's balanced buffer solution (HBBS) was injected into the spleen and 4 months later AHF was induced. Group IV: HBBS was injected into the spleen and AHF was induced at the same time. We evaluated mortality rates, blood glucose levels and histological appearance of the spleen. All animals in groups II, III and IV died in five days whereas two animals died and five animals survived in group I. In group I, blood glucose levels were significantly higher than the others.Macroscopic nodules of hepatocytes and microscopically organized hepatocytes were seen in spleen only in group I. The significant improvement of survival rates in group I compared to groups II- III-IV is due to the net support provided by functioning hepatocytes.
We therefore conclude that FHc transplantation provides metabolic support and improves survival rate if performed prior to AHF.Keywords: Fetal hepatocyte transplantation,
acute hepatic failureTurkiye Klinikleri J Med Sci 1999, 19:85-89
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