ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Carrying Capacities Of The Media That Wasmodified With Mitogen And Various Lysates
Fuat DİLMEÇ*, Ali MATUR**, Mehmet KARAKAŞ***, Soner UZUN****
* Arş.Gör.Dr., Çukurova Üniversitesi Tıp Fakültesi Tıbbi Biyoloji AD,
** Prof.Dr., Çukurova Üniversitesi Tıp Fakültesi Tıbbi Biyoloji AD,
*** Yrd.Doç.Dr., Çukurova Üniversitesi Tıp Fakültesi Dermatoloji AD,
**** Yrd.Doç.Dr., Çukurova Üniversitesi Tıp Fakültesi Dermatoloji AD, ADANA
The molecular methods that can be applied to Leishmania parasites are directly related with the cell number that is present. In this study, it was aimed to increase conventional carrying capacity of culture and to decrease the period to reach the maximum cell number. The medium was prepared as modified Evans' biphasic medium and as monophasic RPMI-1640 with blood or blood products. The determined cell numbers of L.tropica (K-27) and L.donovani (DD-8) were inoculated into these media, seperately.
The 1x RPMI-1640 media of both type, simple and modified ones did not show any positiveness comparing with the preliminary studies performed with traditional media. However, the reaching periods to full capacity happened to be different. L.tropica (K-27) and L.donovani (DD-8) cells in Evans' modified medium and simple 1x RPMI-1640 were highest in 14th, and enriched 1x RPMI-1640 medium with human whole blood lysate reached almost the same level after only four days.
In order to reach the full capacity in minimum period of time, we tried to have the advantage of higher mitotic index with phytohaemagglutinin (PHA). The PHA in different concentrations were added into simple 1x RPMI 1640 media, then the media were inoculated with L.major (5-ASKH). Following the cell numbers throughout the cultivation no positive effect was detected at all.Keywords: Blood lysate, Growth rate, Leishmania culture,
Maximum carrying capacity, Mitogen agentsTurkiye Klinikleri J Med Sci 1999, 19:330-336
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