ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Effect Of Melatonin On Iron Treatment- Inducedoxidative Damage In Rabbits
Ahmet KOYU*, M.Fehmi ÖZGÜNER*, Sadettin ÇALIŞKAN**, Halit KARACA***, Halis KÖYLÜ****
* Yrd.Doç.Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi Fizyoloji AD,
** Prof.Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi Fizyoloji AD,
*** Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi Fizyoloji AD,
**** Doç.Dr., Süleyman Demirel Üniversitesi Tıp Fakültesi Fizyoloji AD, ISPARTA
Melatonin is an antioxidant hormone and acts to stabilize cell membranes, thereby making them more resistant to oxidative attack. Iron (Fe) causes to free radical damage because it plays important role in different oxidative steps under physiological conditions in the body. Iron ions seem to have a major role in initiation and promotion reactions of intracellular lipid peroxidation.The aim of this study was to investigate whether melatonin has protective effect on the oxidative changes induced by Fe treatment in erythrocytes. Thirty New-Zealand male, five months old rabbits weighing 600-800 g were used in this study. The animals were divided into three groups. First group (n:10) was given 500 mg/kg iron-dextran with intraperitoneal injection. Second group was given 500 mg/kg iron-dextran + 100 mg/kg melatonin. Third group was left as controls. Control animals were injected with saline solution. The activities of erythrocyte antioxidant enzymes; Superoxide Dismutase (SOD), Glutathione peroxidase (GSH-Px), Catalase (CAT) and Malondialdehyde (MDA) levels, an indicator of lipid peroxidation, were determined. Erythrocyte SOD, GSH-Px and CAT activities were decreased and MDA levels were increased in iron-dextran treated animals as compared to control group (p<0.05). The activities of three antioxidant enzymes were increased and MDA levels were decreased in iron-dextran and melatonin treated group compared to first group (p<0.05).
Our data indicate that lipid peroxidation occurs after iron overload in the blood. And also, in the light of our findings; melatonin administration can prevent these oxidative toxic effects induced by iron-dependent free radical damage in erythrocytes. This study may be useful for physicians who use iron management in their clinical practice.Keywords: Melatonin ,Fe treatment ,oxidatif demageTurkiye Klinikleri J Med Sci 2000, 20:143-148
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