ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Effects Of Different Culture Andincubation Models On Oocyt In-vitro Maturation
Mehmet CINCIK *, Şaban SEZEN**, Ömer COŞKUN**, Cem KORKMAZ***, H. Mesut ÖZSOY****
* Yrd.Doç.Dr., GATA Tıbbi Histoloji ve Embriyoloji AD,** Uz.Dr., GATA Tıbbi Histoloji ve Embriyoloji AD,*** Dr., GATA Tıbbi Histoloji ve Embriyoloji AD,**** Yrd.Doç.Dr., GATA Kadın Hastalıkları ve Doğum AD, ANKARA
The best culture models and serum support for oocyt culture by combining different incubation models to research in-vitro maturation (IVM) success have been tried to be found. By using controlled ovarian hyperstimulation (COH) immature prophase I (PI) and metaphase I (MI) oocytes (n=970) taken from 120 patients who applied to GATA IVF center in different periods in one year were cultured in various time and conditions. The number and rate of mature oocytes which could reach to metaphase II (MII) in different culture and incubation were determined.
At the incubator conditions of 37 °C, 75-98% humidity and 5% CO2 (pH:7.4), the cultural environments produced by adding 10-15% fetal cord serum (FCS) to the different media are found as the most successful model, increasing the mature oocytes number that could complete their maturity.Keywords: Oocyt, In-vitro maturation,
medium, IncubationTurkiye Klinikleri J Med Sci 2000, 20:291-294
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