ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
The Role Of Oxidative Damage Induced By Endosulfan Toxicity On Rat Liver And Kidney Tissues; A Histological And Biochemical Study
Erdal KARAÖZ*, Tuncer ETYEMEZ**, Mehmet AKDOĞAN***, Alpaslan GÖKÇİMEN****,
Meral ÖNCÜ**, F.Eda AKALIN*****
* Doç.Dr. Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD,
** Dr. Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD,
*** Yrd.Doç.Dr. Süleyman Demirel Üniversitesi Tıp Fakültesi, Biyokimya AD,
**** Yrd.Doç.Dr. Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD,
***** Bio. Süleyman Demirel Üniversitesi Tıp Fakültesi, Histoloji ve Embriyoloji AD, ISPARTA
The histological and biochemical roles of oxidative damage were investigated in relation to hepatotoxicity and nephrotoxicity induced by endosulfan an organochlorine insecticide, in rats. The experimental groups in present study were as folllows: Control (Group I), low dose of endosulfan (Group II), medium dose of endosulfan (Group III) and high dose of endosulfan (Group IV). A single dose was administered via intragastric way to 18 rats in each treatment group at the following levels: 11 mg/kg (10% of LD50) for Group II, 22 mg/kg (20% of LD50) for Group III and 33 mg/kg (30% of LD50) for Group IV, respectively. An equal amount of physiologic saline was also given to control rats. The rats in all treatment groups were killed by cervical dislocation after 24 hours. The tissue samples of liver and kidney were taken by using appropriate techniques for biochemical and histological examination. The level of malondialdehyte (MDA) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione reductase (GR), catalase (CAT) and glucose-6-phosphate dehydrogenase (G6PDH) were determined in tissue samples. Furthermore, tissue samples were kept in 10% neutral formaline solution and then transferred into the paraffine blocks for histological examination. Paraffine sections was stained with Haematoxylen-Eosine and Masson triplet then examined by light microscope.
In this study, a significant increase in liver SOD activity was statistically significant, p<0.01, p<0.01, p<0.01, p<0.05 and between the groups of I and II, I and IV, II and IV, and III and IV, respectively. The increases in kidney SOD activity were also found to be significant, p<0.05, p<0.01 and p<0.05 between the groups of I and III, I and IV, and II and IV, respectively. MDA levels in both kidney and liver increased due to increasing dose of endosulfan. No changes were observed in the activities of GRIn conclusion, an increase in MDA level and SOD activity in rat liver and kidney tissues due to endosulfan may be an indicator of the free radicals occured during the metabolism and their lipid peroxidative inducing damage. Finally, a peroxidative damage occurs inevitably due to endosulfan. The histological results also showed such a damage, similar with the biochemical results. In liver, dilatation in sinuzoidal capillaries, hidropic and vacuolar degenerations in the hepatocytes and Kupffer cell proliferation was examined. The microscopical results in the kidney were mainly perivascular and peritubular mononuclear cell infiltrations, glomerular and tubular degenerations.Keywords: Biochemistry, Endosulfan, anatomy & histology, RatsTurkiye Klinikleri J Med Sci 2001, 21:1-10
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