ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Oxidative Status And Myelin Basic Protein Levels In Experimental Allergic Encephalomyelitis
Filiz Kuralay*, M. Örmen*, P. Akan*, K. Genç**,
S. ÇalIşkan*, M.FadIloğlu*, S. FadIloğlu***
* Dept. of Biochemistry, Medical School of Dokuz Eylul University,
** Dept. of Physiology, Medical School of Dokuz Eylul University,
*** Dept. of Neurology, Medical School of Dokuz Eylul University, İzmir, TURKEY
Purpose: Central nervous system (CNS) tissue is particularly vulnerable to oxidative damage, suggesting that this could be an important factor in the pathogenesis of CNS inflammation in multiple sclerosis (MS) and its model experimental allergic encephalomyelitis (EAE). Free radical production is thought to be involved in inflammatory processes, both exacerbating inflammation and effecting tissue damage. Nitric oxide (NO) and thiobarbituric acid reactive substances mediated lipid peroxidation may be involved in myelin damage in EAE. We therefore examined indirect markers of oxidative stress in EAE. As an index of oxidative alteration, we measured malondialdehyde (MDA) levels in plasma as marker of lipid peroxidation and plasma levels of nitrite and nitrate, the end products of the L-arginine-NO pathway.
Materials and Methods: This study was undertaken at Dokuz Eylül University, Medical School Multidisciplinary Laboratories. Nitrite was measured by using the Griess reaction and nitrate levels were determined by the method of Bories. As a marker of myelin degradation which indicate escape of this myelin component out of the brain, blood myelin basic protein (MBP) levels were determined by RIA method. Blood MDA levels were measured according to the methods of Stocks and Dormandy.
Results: There was no statistically significant elevation in blood MDA or MBP in EAE. There were marked increases in nitrite and nitrate levels.
Conclusion: The reasons for having insignificant results for blood MBP concentrations in EAE could be explained as a consequence of rapid clearance by kidney. These findings may be significant since in comparison to MDA, the end products of the L-arginine-NO pathway, nitrite and nitrate, could be a more important effector molecule in the immunopathogenesis and sistemic activity of MS.Keywords: Multiple sclerosis, Encephalomyelitis, Autoimmune, Experimental, nitric oxide, malondialdehyde, Myelin Basic ProteinsTurkiye Klinikleri J Med Sci 2001, 21:108-113
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