ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Effects Of Cumulus Oophorus Cells On In Vitro Oocyte Maturation
Mehmet CINCIK*, Şaban SEZEN**, H. Mesut ÖZSOY***, Tansu KÜÇÜK****, Cem KORKMAZ*****
* Yrd.Doç.Dr., GATA Tıbbi Histoloji ve Embriyoloji AD,** Uzm.Dr., GATA Tıbbi Histoloji ve Embriyoloji AD,*** Yrd.Doç.Dr., GATA Kadın Hastalıkları ve Doğum AD,**** Doç.Dr., GATA Kadın Hastalıkları ve Doğum AD,***** Dr., GATA Tıbbi Histoloji ve Embriyoloji AD ANKARA
The objective of this study was to investigate the effects of cumulus oophorus cells on in vitro oocyte maturation. A total of 180 oocytes (80 immature - prophase I and 100 intermediate - metaphase I) were collected from 40 patients. These oocytes were randomly distributed into 2 groups.
The first group oocytes were denuded and seperated from cumulus oophorus cells (cumulus free). No denudation was done in the second group. During the incubation period the stage of the in vitro maturation of the cumulus free and intact oocytes were observed every 4 hours up to 36 hours. The undenuded (cumulus intact) oocytes in the first group, were found to reach metaphase II stage 60% (54/90) whereas, denuded oocytes reached 24% (22/90). The peak for reaching metaphase II stage were observed in the undenuded group of oocytes at 12-16 hours.
It is obvious that the presence cumulus oophorus cells play a very important role for in-vitro maturation.Keywords: Oocyte, Cumulus oophorus,
In-vitro oocyte maturationTurkiye Klinikleri J Med Sci 2001, 21:161-165
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