ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
Analysis Of Cardiac Effects Of Short Term And High Dose Growh Hormone Treatment In Rat
Kemal ÖZBİLGİN*, Şenol COŞKUN**, Seda VATANSEVER***, Betül ERSOY****, İbrahim TUĞLU***, Ayşegül UYSAL*****, Ali ONAĞ******
* Doç.Dr.,Celal Bayar Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD,
** Yrd.Doç.Dr.,Celal Bayar Üniversitesi Tıp Fakültesi Çocuk Sağlığı ve Hastalıkları AD,
*** Yrd.Doç.Dr.,Celal Bayar Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD,
**** Doç.Dr., Celal Bayar Üniversitesi Tıp Fakültesi Çocuk Sağlığı ve Hastalıkları AD, MANİSA
***** Doç.Dr., Ege Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD, İZMİR
****** Prof.Dr., Celal Bayar Üniversitesi Tıp Fakültesi Çocuk Sağlığı ve Hastalıkları AD, MANİSA
Purpose: GH is not just related to growth during childhood but also plays an important role for the regulation of body metabolism and growth of many organs. Heart is the most affected organ by applications of GH. Recent years, it has been reported that short-term application of GH might be useful for the treatment of many cardiac disease. Unfortunately, the effect of GH applications on the cardiac tissue is not known completely yet. The, aim of this study is the investigation of the alterations of short-term and high dose of GH application on the cardiac muscle cell and vessels.
Material and Methods: 3 months aged 30 wistar rat are divided into equal 3 groups. First group was treated with 0.3 mg/kg/day GH, and second group was used as a control, which was treatment of equal volume of serum physiologic(SP), applied by intraepidermal single dose-per day for ten days. Nothing was applied to third group(C). Animals were sacrificed at the end of the experiment and their hearts were removed. Tissues were processed for histochemistry, immunohistochemistry and electron microscopy.
Results: There was no difference of the volume of the coronary arteries (C: 9.7±1.2, SP: 9.4±0.9, GH: 11.6±1.1 cm3), the endothelial diameter of the coronary vessels (C:0.64±0.15, SP:0.60±0.17, GH: 0.73±0.014 mm), the endothelial thickness of the coronary arteries (C: 0.004±0.001, SP: 0.004±0.001, GH: 0.005±0.001 mm) and the thickness of the coronary arteries (C: 0.015±0.004, SP: 0.016±0.003, GH: 0.014±0.004 mm) among three groups for tissue, vessels and basal membrane architecture of heart at light microscopic level. However showed that there was endothelial hypertrophy (C: 9.7±6.5, GH: 26.3±9.8 mm, p< 0.0001), occlusion (C: 79.7±43.4, GH: 59.4±21.8 mm, p< 0.016), and Conclusion: These ultrastructural alterations for the effect of GH on the capillaries were seen starting from tenth day. Therefore, it was concluded that patients who were treated with GH should be carefully followed for a long time period because of the possible complications.Keywords: Growth hormone, Heart, Endothelium,
Ultra Structure, Histochemistry,
ImmunohistochemistryTurkiye Klinikleri J Med Sci 2001, 21:173-179
|
|
|
|
|
