ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
|
|
|
|
ORIGINAL RESEARCH ARTICLES |
Regional Differences Of Damage In The Retina After Ischemia
Kemal ÖZBİLGİN*, Barış TOPRAK**, İbrahim TUĞLU***, Verda TOPRAK****, Mine YURTSEVEN*****
* Doç.Dr., Celal Bayar Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD,
** Uz.Dr., Celal Bayar Üniversitesi Tıp Fakültesi Göz Hastalıkları AD,
*** Yrd.Doç.Dr., Celal Bayar Üniversitesi Tıp Fakültesi Histoloji-Embriyoloji AD,
Purpose: Retinal pathology due to ischemia may result with blind eye. Alterations in ischemic retina depends on differences in ischemic duration and magnitude. To know the alterations in the retina due to ischemic injury may help to explain the pathogenic mechanisms and to improve the prevention of the damage. In this study, we aimed to investigate the effect of ischemia on the cells of retina and the alterations of the regional differences in ischemic retina.
Materials and Methods: Twenty male rats were divided into two equal groups. In the first group, intraocular pressure was raised to 150 mmHg with cannulation of the anterior chamber. In the second group (sham) cannulation was performed but the eye was not pressurised. The left eyes of each animal were served as controls. Immediately after the experimental procedure, the animals were sacrificed and the eyes were enucleated for electron microscopic and light microscopic evaluation. The specimens for the light microscopic evaluation were stained with Hematoxilen-Eosin. The thickness of the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer nuclear layer, and photoreceptor layer were measured in the periphery and the central part of the retina and their ratio to total thickness of the retina was calculated.
Results: It was found that the inner plexiform layer was significantly (p<0.05) thinner in the experimental group than the sham group. The peripheral inner plexiform layer was more significantly (p<0.001) thinner than the central inner plexiform layer in the experimental group. The cell morphology was found as normal in the ganglion cell layer and outer nuclear layer. However, it was interesting to note that a few cells in inner nuclear layer were determined as apoptotic according to ultrastructural criteria.
Conclusion: It was concluded that the ischemic damage occurred as thinning of inner plexiform layer was more effective in the peripheral retina and the reason for this result may due to death of cells in the inner plexiform layer by apoptotosis. This result is important for the early diagnosis and treatment of ischemic damage in the retina.Keywords: Ischemia, Retina, Apopitosis, UltrastructureTurkiye Klinikleri J Med Sci 2001, 21:264-269
|
|
|
|
|
