ISSN: 1300-0292
İndekslendiği Dizinler: SCIENCE CITATION INDEX EXPANDED
CINAHL, Index Copernicus,
Chemical Abstracts (CA),
Excerpta Medica / EMBASE
Dil: Türkçe, İngilizce
İçerik: Orijinal Araştırma, Derleme, Editöre Mektup, Olgu Sunumu, Tıp Eğitimi, Tıbbi Kitap İncelemeleri
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ORIGINAL RESEARCH ARTICLES |
Demonstration Of Laminin And Collagen Iv Immunoreactivities Inmouse Retina By Phase Contrast, Normansky And Fluorescence Objectives
H. Seda VATANSEVER*, V. Sevinç İNAN**, Özcan KAYIKÇIOĞLU***, M. Kemal ÖZBİLGİN*
* Yrd.Doç.Dr., Celal Bayar Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji AD,
** Uz.Dr., Celal Bayar Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji AD,
*** Yrd.Doç.Dr., Celal Bayar Üniversitesi, Tıp Fakültesi, Göz Hastalıkları AD, MANİSA
Formation and differences of basement membranes has been provided by their structural proteins. Widely distribution of basement membranes in body, play important roles during cell proliferation, differentiation, migration and function via basement membrane proteins. Retina, which is one of the eye layer, have a specific role during vision with consisting of different cells and basement membrane.
This study was planned to investigate of distribution and importance of laminin and collagen IV that are structural proteins of basement membrane in retina. For this reason, eyes which were collected from six adult mouse were fixed with 4% paraformaldehyde and obtained frozen blocks. Sections (7 m) were stained using indirect immunofluorescence with anti-laminin or anti collagen IV antibodies as a primer antibodies and anti-rabbit TRITC as a secondary antibody. Sections were observed under phase, Normansky and fluorescence objectives.
All layers of retina were detected clearly using both phase and Normansky objectives. Immunoreactivities of both laminin and collagen IV were observed all cell layers of retina and there was no differences of their immunoreactivities.
Our results suggested that function of both proteins in retina was similar because of similar distribution of both basement membrane proteins.Keywords: Basement membrane, Retina, Light microscopyTurkiye Klinikleri J Med Sci 2001, 21:260-263
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